This nudged the second-year graduate student to more investigation: Was vaping as healthy as manufacturers claimed?

For Fernandez, as well as four master’s in biomedicine candidates at ˛ÝÝ®ÉçÇř who defended their theses on Dec. 5, the research process was much more than the sum of the parts.

“I appreciated applying scientific concepts to real world questions that were medically and socially relevant,” said Fernandez, who has received acceptance letters from two medical schools for next year.

Independent research guided by professors

Professor , Fernandez’s advisor, teaches the summer research methods and the fall data methods courses that guide students through the research project, which is the culmination of the five-semester course of study.

“In the summer, the students develop their topic, go through the Institutional Review Board process, create methods and gather data,” she explained. “In the fall class, we look at data analysis and the writing of the manuscript.”

A committee of three professors from different disciplines guides each student through the rigors of research. “We advise carefully, because this is a big investment of time and energy from the student, and an important learning experience,” Halterman said. “You really don’t know how to do research until you create a project of your own and go through the process. There’s troubleshooting, and navigation of politics of different institutions and organizations, and learning how to work with people.”

The process-oriented, hands-on approach is excellent preparation for careers in biomedicine or for medical school, Halterman says. “Most students don’t have any experience with original hypothesis-driven research and this really makes them stand out when they apply to medical or dental school.”

The research project is the final component of a four-part program that includes coursework, a cross-cultural experience and a practicum experience. Transdisciplinary approaches to problem-solving are used and encouraged as students and faculty engage in a curriculum that integrates the natural and medical sciences with faith and ethics, Halterman says, a unique training that also positions students to be collaborative and holistic investigators.

Fall 2015 Research Projects

Rachel Thomas: “A Data Analysis on Demographic Information of Patients with ADHD at [local medical practice]”

Thomas, who works in healthcare and has a goal of becoming a physician’s assistant, says her interest arose from her own awareness of the high rates of ADHD diagnosis. She analyzed demographical data from an area medical practice to identify correlations between adult ADHS and gender, race/ethnicity and socioeconomic status. In demographical analyses of childhood ADHD already done by other researchers, ADHD was more frequently observed in Caucasians and in males. The data set included more than 970 patients ranging in age from 18-65+ and confirmed that a diagnosis of adult ADHS was more likely to be seen in Caucasion men than in other populations. Her research will be used to help identify and treat adult patients with ADHD. Committee members: Drs. and

Hannah Bell: “Women’s Awareness of Risk Factors Linked to Breast Cancer in Harrisonburg, Virginia”

Bell conducted a survey in which 60 female respondents self-reported their knowledge about obesity and breast cancer. She found that women in Harrisonburg did not fully understand the risk factors of breast cancer, including the role of postmenopausal obesity. Though the survey only covered a small population, it affirmed the need to raise awareness among the local population about the risk factors. Committee members: Drs. and

Vipul Nayani:  “The Effect of Collegiate Athletic Training Duration on Athlete Injury Incidence”

Nayani, a 2014 Virginia Tech graduate who plans to become a family physician, developed this project with an interest in “multifactorial” causes and individual proclivities toward injury. He observed a correlation between training time and injury, with evidence suggesting that more than 18 hours of weekly athletic training were 23% more likely to develop athletic injuries than athletes that trained for fewer than 18 hours per week. Committee Members: Drs. and Carolyn Stauffer.

Tareq Yousef: “Cost Analysis of Dental Services Before and After the Implementation of the Affordable Care Act in the Washington, D.C. Metropolitan Area and the Shenandoah Valley Region of Virginia”

Yousef, who has an undergraduate degree from George Washington University in public health, wanted a research topic that integrated current political events with healthcare, and specifically dentistry, his future profession. He studied the effect of the Medical Device Excise Tax, which supports the Affordable Care Act and is levied on device manufacturers, on the cost of oral health services in dental offices and labs. Statistically significant changes in cost of services were reported for all of the lab services as well as some dental procedures. Committee members: Drs. and Roman Miller.

Jared Fernandez: “Carcinogenic Compounds Created by the Vaporization of Electronic Cigarette Liquid: Effects of Variable Voltage within the Modified Electronic Cigarette”

Fernandez’s research, outlined briefly above, hypothesized that there would be positive correlation between the voltage setting of the modified E-cig and the amount of carcinogens within the vapor produced from one modified E-cig design. While no detectable amounts of carcinogens were produced in his data set, Fernandez found that the content of the vapor is significantly different depending on the voltage. Furthermore, increased voltage may increase inflammatory responses in human lungs regardless of flavoring additives due to increased concentrations within inhaled vapors. These findings suggest additional research is required to further characterize E-cig effects on human health. Committee members: Drs. and .

Eight presentations were made by 14 biology, , and students that showcased the breadth and depth of research opportunities available at EMU.

The symposium provided an opportunity for students to share their research with an audience of general science majors, said department chair and professor of chemistry . One of the skills of a scientist, she says, is “to be able to present technical terminology and processes in accessible language. It’s great if a scientist can study climate change, for example, but if we can’t explain the results of a climate change study to someone who doesn’t have the background, that doesn’t help us share and discuss our findings.”

The evaluation – led by , professor of chemistry at James Madison University – included three criteria: collection of data, thoroughness of research design and analysis, and clarity and accessibility of the oral presentation.

Second place was awarded to junior Hannah Daley for her presentation about her National Science Foundation-sponsored research, in partnership with two professors at James Madison University, on the effects of caffeine on athletes.

Sophomore Kat Lehman and junior Diego Barahona were awarded third place for their research on utilizing radio-tracking to locate coconut rhinoceros beetles’ breeding sites, which was conducted under the direction of chemistry professor in Guam. Siderhurst has worked for several years on research projects involving the development of attractants for invasive and agriculturally important pests.

Students find their niche in grant-funded studies

Research projects often pair students with professors engaged in long-term studies. The competition winners, Ferguson and Fernandez, were research assistants to Professor , who earned a $100,000 Jeffress Trust grant to study a multi-year investigation into the causes of hypertension. In Ferguson and Fernandez’s project, rats were fed a high fructose, high salt, low salt, and no salt diet and measured the activation of genes involved in disease.

The STEM symposium is also offers students like Hannah Daley and Janaya Sachs, who both earned grants to engage research opportunities off campus, to share what they’ve learned. Sachs explored a new ligand for synthesizing copper, a summer project she engaged in through the National Science Foundation Research Experience for Undergraduates. She worked at .

The bioorganic copper project is just one of several research opportunities she’s been involved in. Her freshman year, she joined , led by , director of the MA in biomedicine program, and supported by a grant from the U.S. Department of Agriculture. She and Hannah Daley are both currently involved in evaluating the effects of hydrofracking on stream and well water, a project under the direction of and Kishbaugh.

Among the research shared, a unique transdisciplinary project was conducted by biology major Samuel Miller and computer science major Alex Bender, advised by Siderhurst and computer science professor . Miller conducted a chemistry project that involved identification and quantification of volatiles released by the noni fruit during ripening that provided a complex data set that was difficult to analyze. Bender and Miller have both used a mathematical technique called Principal Component Analysis to analyze the results in a simpler manner.  Bender is documenting their methodology for PCA usage, simultaneously evaluating software programs Cluster 3.0 and SPSS for their ability to simplify the data set.

Flavenoids, genetics, mold…

Professor , who teaches courses in genetics, cell biology, microbiology and immunology, advised several research projects. Biology major Chris Miller researched the effect of anthocyanins, richly concentrated plant colorants that may have protective effects, on mouse fetal development when the mice are exposed to a significant amount of alcohol.

Derek Harnish and Eli Wenger presented their research on the neuronal basis of aging in flies. Harnish, a biology major, and Wenger, a biology and biochemistry double major, worked with Copeland on his ongoing project to identify specific types of that have the potential to extend lifespan in flies when the mitochrondrial electron transport chain is disrupted.

Biology majors Viktor Kaltenstein and Seth Suttles teamed up to study mold growth in a vacated section of the Suter Science Center campus using the Environmental Relative Moldiness Index (ERMI).